Description
T7 Endonuclease I is a junction-resolving enzyme of 149 amino acid residue, encoded by gene 3 of bacteriophage T7, existing as a stable dimer. Not only does it selectively bind and cleave four-way DNA (Holliday) junctions with high specificity for branched structures in double-stranded DNA, such as cruciform DNA, but also it has a strong preference for cutting single-stranded DNA. It requires metal ions such as magnesium for activity. This product is purified from E. coli expressing the recombinant T7 Endonuclease I (T7EI) gene